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Construction of a bacterial artificial chromosome library containing large Eco RI and Hin dIII genomic fragments of lettuce

โœ Scribed by A. C. J. Frijters; Z. Zhang; M. van Damme; G.-L. Wang; P. C. Ronald; R. W. Michelmore


Publisher
Springer
Year
1997
Tongue
English
Weight
386 KB
Volume
94
Category
Article
ISSN
0040-5752

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A yeast artificial chromosome (YAC) genomic library of Beta vulgaris was constructed in the pYAC4 vector. High-molecular-weight DNA was prepared from agarose-embedded leaf protoplasts from a triploid cultivar. The library was found to contain 33,500 clones in an ordered array of microtiter plates. M