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Construction in vitro of “Phage-plasmid” chimerae: a new tool to analyse the mechanism of plasmid maintenance

✍ Scribed by Couturier, M. ;Janssens, J. ;Bex, F. ;Desmyter, A. ;Bonnevalle, I.

Publisher: Springer
Year: 1979
Tongue: English
Weight: 327 KB
Volume: 169
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00267552

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✦ Synopsis

In this paper, we report the construction in vitro of chimerae between lambdoid replacement vectors (Murray et al., 1977) and the miniF Apr plasmid: pSC138 (Timmis et al., 1975). lambdaF recombinants were shown to be chimerae between the lambda and the F replicons. By genetical tests, we have demonstrated that both lambda and F replication mechanisms are functional: the lambdaF recombinant behaves as a non defective plaque forming phage on lambda sensitive bacteria and establishes itself as a stable plasmid on recA F- homoimmune bacteria. In the extra-chromosomal state, the lambdaF recombinant apparently retains the controlled autonomous replication and the FI incompatibility characteristics of the F plasmid. The potential experimental uses of these phages are discussed.

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