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Construction and evaluation of a frequency-domain epifluorescence microscope for lifetime and anisotropy decay measurements in subcellular domains

✍ Scribed by A.S. Verkman; Michael Armijo; Kiyohide Fushimi


Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
841 KB
Volume
40
Category
Article
ISSN
0301-4622

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Optimized protocol of a frequency domain
✍ Aymeric Leray; Franck B. Riquet; Elodie Richard; Corentin Spriet; Dave Trinel; L 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 511 KB

## Abstract Frequency‐domain fluorescence lifetime imaging microscopy (FLIM) has become a commonly used technique to measure lifetimes in biological systems. However, lifetime measurements are strongly dependent on numerous experimental parameters. Here, we describe a complete calibration and chara