Optimized protocol of a frequency domain
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Aymeric Leray; Franck B. Riquet; Elodie Richard; Corentin Spriet; Dave Trinel; L
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Article
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2009
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John Wiley and Sons
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English
⚖ 511 KB
## Abstract Frequency‐domain fluorescence lifetime imaging microscopy (FLIM) has become a commonly used technique to measure lifetimes in biological systems. However, lifetime measurements are strongly dependent on numerous experimental parameters. Here, we describe a complete calibration and chara