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Construction and detection of expression vectors of microRNA-9a in BmN cells

โœ Scribed by Yong Huang; Quan Zou; Sheng-peng Wang; Shun-ming Tang; Guo-zheng Zhang; Xing-jia Shen

Book ID
111842937
Publisher
SP Zhejiang University Press
Year
2011
Tongue
English
Weight
578 KB
Volume
12
Category
Article
ISSN
1673-1581

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A strong transcriptional signal previously cloned from the Streptomyces griseus genome in S. lividans was subcloned and its nucleotide sequence was determined. Upstream of the transcriptional start point which was determined by high-resolution S1 nuclease mapping, -35 (5'-TTGCCG-3') and -10 (5'-TAGC