Construction and characterization of double mutants in nitrogenase ofKlebsiella pneumoniae
β Scribed by Dehua Zhao; Jilun Li
- Book ID
- 105728902
- Publisher
- Springer
- Year
- 2004
- Tongue
- English
- Weight
- 677 KB
- Volume
- 49
- Category
- Article
- ISSN
- 1001-6538
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The structural genes coding for the two kinds of subunits of phosphofructokinase in yeast have been cloned previously (Heinisch 1986). The coding regions were defined by S1-mapping. They were disrupted in vitro by insertion of a LEU2-marker. These constructions were then used for substitution of the