Construction and Characterization of a New Flexible and Nonbreakable Nitric Oxide Microsensor

✍ Scribed by Alec Dickson; Jie Lin; Jie Sun; Mark Broderick; Harry Fein; Xueji Zhang

• Publisher: John Wiley and Sons
• Year: 2004
• Tongue: English
• Weight: 84 KB
• Volume: 16
• Category: Article
• ISSN: 1040-0397
• DOI: 10.1002/elan.200302852

✦ Synopsis

Abstract

Nitric oxide (NO) is an important molecule in many different physiological phenomena. Investigation of nitric oxide production in vivo requires a specialized sensor capable of real‐time concentration measurement, with a high spatial resolution of NO gas production. In this study, a flexible microsensor is developed specifically for measurement of production of nitric oxide. The new sensor consists of a Pt/Ir working electrode coupled with an integrated Ag/AgCl reference electrode. The sensor is coated with a series of NO‐selective membrane polymers to prevent potential amperometric response due to interfering species. Presented experimental data demonstrates the ability for NO detection between 100 and 400 nM concentrations with a linear response (R^2^=0.9997). The detection limit of the sensor is 2.14 nM (S/N=3). Various selectivity experiments are indicative of a resistance to interfering species such as dopamine, norepinephrine, L‐arginine.