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Construction and characterization of a large insert bovine YAC library with five-fold genomic coverage

✍ Scribed by T. P. L. Smith, L. J. Alexander, T. S. Sonstegard, J. Yoo, C. W. Beattie, M. F. Broom


Book ID
113034844
Publisher
Springer-Verlag
Year
1996
Tongue
English
Weight
870 KB
Volume
7
Category
Article
ISSN
0938-8990

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Gridded genomic libraries are crucial for the positional candidate gene approach. For this purpose we constructed a gridded genomic library from a female chicken using the vector sCos 1. About 110 000 cosmid clones were grown and replicated in 384‐well plates. An average insert size of 39 kb was cal