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Construction and Characterization of a 10-Genome Equivalent Yeast Artificial Chromosome Library for the Laboratory Rat,Rattus norvegicus

✍ Scribed by Li Cai; Leonard C. Schalkwyk; Andreina Schoeberlein-Stehli; Robert Y.L. Zee; Avrial Smith; Thomas Haaf; Michel Georges; Hans Lehrach; Klaus Lindpaintner


Book ID
115613631
Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
556 KB
Volume
39
Category
Article
ISSN
0888-7543

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✍ J. Del-Favero; M. Vauterin; G. Weyens; K. E. Edwards; M. Jacobs πŸ“‚ Article πŸ“… 1994 πŸ› Springer 🌐 English βš– 622 KB

A yeast artificial chromosome (YAC) genomic library of Beta vulgaris was constructed in the pYAC4 vector. High-molecular-weight DNA was prepared from agarose-embedded leaf protoplasts from a triploid cultivar. The library was found to contain 33,500 clones in an ordered array of microtiter plates. M