The production of endo-beta-1,4-glucanase by a Bacillus strain isolated from a hot spring in Zimbabwe was studied in batch culture, chemostat culture, and carbon dioxide-regulated auxostat (CO2-auxostat). The bacteria produced the enzyme in the presence of excess glucose or sucrose, but not under ca
Constitutive production of endoglucanase by aBacillussp. isolated from a Zimbabwean hot spring
โ Scribed by R. Zvauya; C. J. Zvidzai
- Publisher
- Springer
- Year
- 1995
- Tongue
- English
- Weight
- 208 KB
- Volume
- 11
- Category
- Article
- ISSN
- 1573-0972
No coin nor oath required. For personal study only.
โฆ Synopsis
A sporulating, aerobic Bacillus sp., isolated from Chimanimani hot springs, Zimbabwe, produced endoglucanase when cultured on medium with initial pH between 5.0 and 9.0 and at 30 to 60ยฐC. Optimal production of endoglucanase was at pH 6.0. The enzyme was constitutively produced when the organism was cultured on starch, cellobiose, carboxymethylcellulose, sucrose, glucose, galactose, Avicel, lactose, mannose or maltose.
๐ SIMILAR VOLUMES
Two Bacillus strains capable of producing endo-p-1,4-glucanase enzyme were isolated from Zimbabwean hot springs. Strain HR68 produced the enzyme when cultured using both carboxymethylcellulose (CMC) and simple sugars as carbon source while strain CH43 produced the enzyme on CMC only. The optimum con