Constant and variable parts of the 155-bp centromeric repeat inCamptochironomus

✍ Scribed by Ching Liao; Carlos Rovira; Jan -Erik Edström

• Publisher: Springer
• Year: 1994
• Tongue: English
• Weight: 269 KB
• Volume: 39
• Category: Article
• ISSN: 0022-2844
• DOI: 10.1007/bf00178257

✦ Synopsis

Centromeres in dipteran insects belonging to the subgenus Camptochironomus (C. tentans and C. pallidivittatus) contain tandem repeats of a 155-bp repeat. A special structural feature of the 155-bp unit is a region with two palindromes connected by a short piece of DNA with only AT base pairs, which has at least a superficial similarity to a functionally important part of the Saccharomyces cerevisiae centromere. As a parameter for functional importance we have measured frequencies of mutations along the unit in samples of repeats from the two species. We find that it consists of about equal parts of highly conserved and considerably less-conserved DNA. The palindromes are localized in the conserved part of the repeat.