Conserved V(D)J junctional sequence of cross-reactive cytotoxic T cell receptor idiotype and the effect of a single amino acid substitution

✍ Scribed by Toshiyasu Hirama; Sunao Takeshita; Yuji Matsubayashi; Michihiro Iwashiro; Tohru Masuda; Kagemasa Kuribayashi; Yataroh Yoshida; Hideo Yamagishi

• Publisher: John Wiley and Sons
• Year: 1991
• Tongue: English
• Weight: 507 KB
• Volume: 21
• Category: Article
• ISSN: 0014-2980
• DOI: 10.1002/eji.1830210234

✦ Synopsis

Introduction

Conserved V(D)J junctional sequence of cross-reactive cytotoxic T cell receptor idiotype and the effect of a single amino acid substitution* A dominant Tcell population bearing the cross-reactive idiotype of Tcell antigen receptor (TcR) has been obtained using an anti-TcR monoclonal antibody (mAb) developed in syngeneic mice. Forty-four cytotoxic T cell (CTL) clones with reactivity to a mAb (N9-127) were selected out of 396 H-2Db-restricted CTL clones specific for FBL-3 tumor antigen from C57BL/6 mice. These CTL clones were divided into two groups according to the blocking pattern of cytotoxic activities with mAb N9-127. All eight CTL clones chosen from both groups expressed TcR with a specific combination of a and p chains (Va1Ja112-WplODp2.1Jp2.7), and the difference in the blocking susceptibility resided in a single amino acid substitution (Gly to Asp) in the D-J joint of p chain. This provides direct evidence for the molecular basis of cross-reactive idiotypes of TcR recognized by mAb.