Conserved sequence pattern in a wide variety of phosphoesterases

✍ Scribed by Eugene V. Koonin

• Book ID: 105356194
• Publisher: Cold Spring Harbor Laboratory Press
• Year: 2008
• Tongue: English
• Weight: 291 KB
• Volume: 3
• Category: Article
• ISSN: 0961-8368
• DOI: 10.1002/pro.5560030218

✦ Synopsis

Abstract

A unique sequence pattern, designated the GD/GNH signature, was shown to be conserved in a wide variety of phosphoesterases. The enzymes containing this signature cleave phosphoester bonds in such different substrates as (1) phosphoserine and phosphothreonine in polypeptides; (2) bis(5′‐nucleosidyl)‐tetraphosphates; (3) nucleoside 5′ phosphates; (4) 2′,3′‐cyclic nucleotide phosphates; (5) polynucleotides; (6) 2′‐5′ phospho‐diesters in RNA (intron) lariats; (7) sphingomyelin; and (7) various phosphomonoesters. Two conserved acidic amino acid residues and a conserved histidine residue may be directly involved in phosphoester bond cleavage.