Conservation of Extended Promoter Regions of Nodulation Genes in Rhizobium
β Scribed by Katalin Rostas, Eva Kondorosi, Beatrix Horvath, Andras Simoncsits and Adam Kondorosi
- Book ID
- 123632377
- Publisher
- National Academy of Sciences
- Year
- 1986
- Tongue
- English
- Weight
- 958 KB
- Volume
- 83
- Category
- Article
- ISSN
- 0027-8424
- DOI
- 10.2307/26872
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Some primitive pea lines, e.g. cultivar Afghanistan, are resistant to nodulation by most strains of Rhizobium leguminosarum. However the Turkish strain TOM can nodulate cv. Afghanistan in addition to commercial pea varieties, and this extended host range is a property of its symbiotic plasmid, pRL5J
A 135 kb long segment of the symbiotic region of the Rhizobium meliloti megaplasmid was mapped with the help of a Rhizobium meliloti gene library, made in the cosmid vehicle pJB8. A set of overlapping cosmid clones was used to identify the inserts in R-primes carrying megaplasmid sections, and to ma