Consecutive demonstration of catecholamines and dopamine-β-hydroxylase within the same specimen

✍ Scribed by R. König

• Book ID: 104789449
• Publisher: Springer
• Year: 1979
• Tongue: English
• Weight: 565 KB
• Volume: 61
• Category: Article
• ISSN: 1432-119X
• DOI: 10.1007/bf00508451

✦ Synopsis

A combined method was developed for characterization and differentiation of catecholamines in neuron populations containing more than one catecholamine, e.g. dopamine and norepinephrine. Its application to small intensely fluorescent (SIF-) cell clusters in sympathetic ganglia allows the successive demonstration of glyoxylic acid-induced catecholamine fluorescence and dopamine-beta-hydroxylase by indirect immunofluorescence within the same tissue section. Applying this technique to an example, two types of SIF-cells were demonstrated in the guinea pig superior cervical ganglion.