Connective tissue activating peptide III induction of synthesis and secretion of plasminogen activator by synovial fibroblasts

✍ Scribed by Carol Godoshian Ragsdale; C. William Castor; Dedra J. Roberts; Kenneth H. Swartz

• Publisher: John Wiley and Sons
• Year: 1984
• Tongue: English
• Weight: 495 KB
• Volume: 27
• Category: Article
• ISSN: 0004-3591
• DOI: 10.1002/art.1780270609

✦ Synopsis

Connective tissue activating peptide I11 (CTAP-111) is a platelet factot that induces, in cultured connective tissue cells, activities observed in chronic inflammation. In this study we measured plasminogen activator secretion by synovial fibroblasts after stimulation by CTAP-111. Increased plasminogen activator secretion was observed 24-48 hours after stimulation. Induction was prevented by dexamethasone ( 10-9-10-7M), cycloheximide (1 pg/ml) and, variably, by aetinomycin D (0.3 pglml), but not by cytosine arabinoside (10-4n/i. This is the first evidence that CTAP-I11 induces degradative as well as proliferative activity by connective tissue cells.

Human platelets contain, and release during degranulation, a 9,278 dalton cationic protein, connective tissue activating peptide 111 (CTAP-1111, which activates normal synovial and skin fibroblasts and chondrocytes (1,2). Such activation results in metabolic and secretory activities characteristic of inflammatory states and detectable in rheumatoid synovial cells in culture. Among its established actions, CTAP-I11 is mitogenic and stimulates glycosaminoglycan forma-From the