Conjugation of siRNA with Comb-Type PEG Enhances Serum Stability and Gene Silencing Efficiency

✍ Scribed by Karthikeyan Gunasekaran; Thi H. Nguyen; Heather D. Maynard; Thomas P. Davis; Volga Bulmus

• Book ID: 102496751
• Publisher: John Wiley and Sons
• Year: 2011
• Tongue: English
• Weight: 471 KB
• Volume: 32
• Category: Article
• ISSN: 1022-1336
• DOI: 10.1002/marc.201000804

✦ Synopsis

Abstract

A thiol‐modified siRNA targeting the enhanced green fluorescence protein (eGFP) gene was conjugated with RAFT‐synthesized, pyridyl disulfide‐functional poly(PEG methyl ether acrylate)s (p(PEGA)s). siRNA‐p(PEGA) conjugates demonstrated significantly enhanced in vitro serum stability and nuclease resistance compared to the unmodified and thiol‐modified siRNA. The complexes of siRNA‐p(PEGA) conjugates with a fusogenic peptide, KALA ((+)/(–) = 2) inhibited the protein expression approximately 28‐fold more than the KALA complex of the unmodified siRNA. The protein inhibition caused by siRNA‐p(PEGA)‐KALA complexes (56 ± 5%–58 ± 3% of the fluorescence expressed in non‐treated cells) was comparable to the effect of the unmodified siRNA‐lipofectamine complex (77 ± 7%). magnified image