Conjugation deficient E. coli K12 F- mutants with heptose-less lipopolysaccharide

✍ Scribed by Havekes, Louis M. ;Lugtenberg, Ben J. J. ;Hoekstra, Wiel P. M.

• Publisher: Springer
• Year: 1976
• Tongue: English
• Weight: 754 KB
• Volume: 146
• Category: Article
• ISSN: 0026-8925
• DOI: 10.1007/bf00267981

✦ Synopsis

Two F- mutants deficient in conjugation with F-type donors are isolated and characterized. Phenotypically, these mutants are similar; they have heptose-less lipopolysaccharide and lack some outer membrane protein. Genotypically, they are different. One mutant harbors a point mutation in the 70 to 74 min region, while the other is deleted for the chromosomal region 6.5 to 8.5 min. Comparison of the properties of the conjugation-deficient mutants described in this paper with other such mutants suggests that an outer membrane protein is the receptor for the f-pilus.