Conjugated α-linolenic acid production from α-linolenic acid by Lactobacillus plantarum AKU 1009a
✍ Scribed by Shigenobu Kishino; Jun Ogawa; Akinori Ando; Sakayu Shimizu
- Publisher
- John Wiley and Sons
- Year
- 2003
- Tongue
- English
- Weight
- 192 KB
- Volume
- 105
- Category
- Article
- ISSN
- 1438-7697
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✦ Synopsis
Abstract
Conjugated α‐linolenic acid (CALA) was produced by incubation of α‐linolenic acid with the washed cells of Lactobacillus plantarum AKU 1009a. The washed cells expressing high levels of CALA productivity were obtained by cultivation in a nutrient medium supplemented with 0.01% (wt/vol) α‐linolenic acid as an inducer. Under the optimum reaction conditions with 63 mg/ml α‐linolenic acid as substrate, the washed cells produced 25 mg CALA/ml reaction mixture (40 mol‐% yield) in 72 h. The produced CALA were a mixture of the two isomers, i.e., cis‐9, trans‐11, cis‐15‐octadecatrienoic acid (CALA1, 67% of total CALA) and trans‐9, trans‐11, cis‐15‐octadecatrienoic acid (CALA2, 33% of total CALA), and accounted for 48% of total fatty acid obtained. Almost stoichiometric conversion was attained with 12 mg/ml α‐linolenic acid as the substrate in 48 h reaction time. It resulted in 12 mg CALA/ml reaction mixture consisting of 43% CALA1 and 57% CALA2 accounting for 66% of total fatty acid obtained. The CALA produced was obtained as the free fatty acid.