Conjugated NAD (H)-dependent Dehydrogenases for the Continuous Production of Mannitol and Gluconic Acid from Glucose-Fructose Mixtures in a Membrane Reactor

Improved operational stability of cell-f

Improved operational stability of cell-free glucose-fructose oxidoreductase from Zymomonas mobilis for the efficient synthesis of sorbitol and gluconic acid in a continuous ultrafiltration membrane reactor

✍ Bernd Nidetzky; Monika Fürlinger; Dorothee Gollhofer; Robert K. Scopes; Dietmar 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 177 KB 👁 2 views

For the continuous, enzymatic synthesis of sorbitol and gluconic acid by cell-free glucose-fructose oxidoreductase (GFOR) from Zymomonas mobilis, the principal determinants of productivity have been identified. Most important, the rapid inactivation of the soluble enzyme during substrate conversion