Conjugal transfer of nopaline and agropine Ti-plasmids —The role of agrocinopines
✍ Scribed by Ellis, J. G. ;Kerr, A. ;Petit, A. ;Tempe, J.
- Publisher
- Springer
- Year
- 1982
- Tongue
- English
- Weight
- 633 KB
- Volume
- 186
- Category
- Article
- ISSN
- 0026-8925
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
Electroporation methods and conjugal matings were used to transfer several plasmid vectors to Aquaspirillum dispar and Aquaspirillum itersonii. The incompatibility P class plasmid RP4 was conjugally transferred from Escherichia coli HB101 to these spirilla, and the transconjugants subsequently donat
The nucleotide sequence of the T-DNA region encoding transcripts 6a and 6b of the pTiT37 nopaline Ti plasmid has been determined. Analysis of this sequence allowed us to find two open reading frames in opposite orientation and separated by 482 bp. Comparison with previous published nucleotide sequen
The origin of transfer (oriT) is the sequence within which conjugal transfer of plasmid DNA is initiated, and is absolutely required in cis for plasmid mobilization. We have cloned oriT from the 52 kb IncN plasmid R46 on a 600 bp fragment, and mapped the limits of the relevant sequence by deletion a