Congenital nemaline myopathy. II. Quantitative changes in α-actinin and myosin in skeletal muscle
✍ Scribed by Dr. Ingo Stuhlfauth; Dr. Frans G. I. Jennekens; Dr. Jacobus Willemse; Dr. Brigitte M. Jockusch
- Publisher
- John Wiley and Sons
- Year
- 1983
- Tongue
- English
- Weight
- 647 KB
- Volume
- 6
- Category
- Article
- ISSN
- 0148-639X
No coin nor oath required. For personal study only.
✦ Synopsis
Skeletal muscle obtained from 2 patients with congenital nernaline rnyopathy (CNM) and from a healthy control was analyzed by 1-and 2dimensional gel electrophoresis. In total extracts, an increase of aactinin by a factor of 2:3 was found for CNM muscle as compared with the control. One-and two-dimensional gels revealed the presence of LCF3, the smallest light chain associated with type 2 (fast) myosin in total extracts of normal control of mixed fiber type. Both CNM samples showed the absence of this polypeptide. This result is consistent with the finding that muscle of the 2 patients exhibited nearly exclusively the ATPase activity indicative of type 1 (slow) myosin.