## Abstract The hydrocarbon‐deoxyribonucleoside adducts formed in mouse skin DNA have been determined following topical application of an initiating dose of benzo(a)pyrene to Swiss mice, a strain shown to be susceptible to benzo(a)pyrene‐induced skin carcinogenesis. Several DNA‐bound products were
Conformations and selective photodissociation of heterogeneous benzo(a)pyrene diol epoxide enantiomer-DNA adducts
✍ Scribed by David Zinger; Nicholas E. Geacintov; Ronald G. Harvey
- Publisher
- Elsevier Science
- Year
- 1987
- Tongue
- English
- Weight
- 736 KB
- Volume
- 27
- Category
- Article
- ISSN
- 0301-4622
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DNA adducts are mainly detected by 32 P-postlabeling and enzyme-linked immunosorbent assays. We have established a method for detection of benzo[a]pyrene-diol-epoxide (BPDE)-DNA adducts by flow cytometry, and have clarified the effects of the DNA adducts on cell-cycle progression and the relationshi
## Abstract Benzo[__a__]pyrene diol epoxide (BPDE) was reacted __in vitro__ with (2'‐deoxy)nucleotides and with calf thymus DNA. The modified DNA was enzymatically hydrolyzed to the 5'‐monophosphate nucleotides using deoxyribonuclease I (DNA‐ase I), nuclease P1 and snake venom phosphodiesterase (SV