Conformational transitions of thioredoxin in guanidine hydrochloride
β Scribed by Kelley, Robert F.; Stellwagen, Earle
- Book ID
- 127382084
- Publisher
- American Chemical Society
- Year
- 1984
- Tongue
- English
- Weight
- 962 KB
- Volume
- 23
- Category
- Article
- ISSN
- 0006-2960
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π SIMILAR VOLUMES
The unfolding and refolding of T4 thioredoxin was observed by equilibrium and kinetic size exclusion chromatographic measurements in guanidine hydrochloride at 4Β°C and pH 7.0. All the observed chromatographic profiles can be simulated by a cubic mechanism using a consistent set of equilibrium and ki
## Abstract By analyzing the effect of urea and guanidine hydrochloride on the circular dichroism of many polypeptides and proteins, it is concluded that under conditions of high concentration of the perturbant and at low temperatures the resultant state approached is that of a local extended helix