𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Conformational studies of bacterial polysaccharides. II. Optical activity of some bacterial capsular polysaccharides

✍ Scribed by Ch. Wolf; U. Elsässer-Beile; S. Stirm; G. G. S. Sutton; W. Burchard

Publisher
Wiley (John Wiley & Sons)
Year
1978
Tongue
English
Weight
875 KB
Volume
17
Category
Article
ISSN
0006-3525

No coin nor oath required. For personal study only.

✦ Synopsis

The optical activity of the Klebsiella capsular polysaccharides of serotypes K1, K5, K6, K8, K11, K56, and K57 has been studied in aqueous solution. Measurements of ORD in the range 185450 nm reveal anomalous ORD with Cotton effects near XO = 195 nm. The results are evaluated quantitatively according to the Moffitt-Yang and the Drude equations. Straight lines are obtained in the Moffitt-Yang plots, while the corresponding Drude plots yield bent curves. The bo values, calculated from the slope of the straight lines in the Moffitt-Yang plot, range from 90 to 270 and suggest a helical superstructure for the capsular polysaccharides. Positive bo values have been found for K1, K5, and K6 and negative bo values for K8, K11, K56, and K57. Circular dichroism has been measured, but the CD curves are found to be truncated a t the lower-wavelength end due to the 185-nm limit of the spectrometer used.

Measurements of the temperature dependence of the specific optical rotation [a] reveal in all cases cooperative orderdisorder transi'tions a t temperatures, T,, from 298 to 323°K. The van't Hoff enthalpies derived from the width of the transition curves are found to be similar in value to those of polypeptides in aqueous solution. The K8 polysaccharide shows a two-step transition. The results are discussed in relation to the known primary structure and x-ray data from oriented and partially crystalline films. A model is suggested for the two-step transition in the K8 polysaccharide. * Part I, see Ref. 29.

📜 SIMILAR VOLUMES

Three-dimensional features of the bacter
Three-dimensional features of the bacterial polysaccharide (1 → 4)-β-D-glucuronan: A molecular modeling study
✍ Isabelle Braccini; Alain Heyraud; Serge Pérez 📂 Article 📅 1998 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 220 KB 👁 1 views

The mutant strain M5N1 CS of Rhizobium meliloti produces, in a Rhizobium complete medium supplemented with fructose and sucrose, a partially acetylated homopolymer of D-glucuronic acid residues linked b-(1 r 4). This polysaccharide forms thermoreversible gels with monovalent salts and thermally stab

Spectroscopic origin of conformation-sen
Spectroscopic origin of conformation-sensitive contributions to polysaccharide optical activity: Vacuum-ultraviolet circular dichroism of agarose
✍ J. N. Liang; E. S. Stevens; E. R. Morris; D. A. Rees 📂 Article 📅 1979 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 401 KB 👁 2 views

## Abstract The vacuum‐uv CD of agarose solid films has been measured to 145 nm and shows a positive band near 180 nm and a larger negative band at around 152 nm. The positive band remains accessible in aqueous solution and has been used to characterize changes in molecular conformation and interac

ChemInform Abstract: First Application o
ChemInform Abstract: First Application of Triflic Acid for Selective Cleavage of Glycosidic Linkages in Structural Studies of a Bacterial Polysaccharide from Pseudoalteromonas sp. KMM 634.
✍ Andrei V. Perepelov; Sof'ya N. Senchenkova; Aleksander S. Shashkov; Nadezhda A. 📂 Article 📅 2010 🏛 John Wiley and Sons ⚖ 30 KB

## Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable v

MO-Calculation of the Optical Activity o
MO-Calculation of the Optical Activity of Oligopeptides. II. Open-chain conformations. Comparison with some cyclic systems
✍ Max Iseli; Rudolf Geiger; Georges Wagnière 📂 Article 📅 1978 🏛 John Wiley and Sons 🌐 German ⚖ 554 KB

## Abstract Using the Frozen Core (FC) MO procedure described in a previous communication, we have computed the long‐wavelength chiroptic properties of oligopeptides in the parallel‐chain (PC) pleated sheet conformation, in the poly (L‐proline) **I** and poly (L‐proline) **II** conformations. The m