Conformational studies of Aib-rich peptides containing lactam-bridged side chains: Evidence of 310-helix formation
✍ Scribed by E. Schievano; K. Pagano; S. Mammi; E. Peggion
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 2005
- Tongue
- English
- Weight
- 260 KB
- Volume
- 80
- Category
- Article
- ISSN
- 0006-3525
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
Aib‐rich side‐chain lactam‐bridged oligomers Ac‐($\rm{G{\overline{lu-Aib-Aib-Ly}s}}$)~n~‐Ala‐OH with n = 1,2,3 were designed and synthesized as putative models of the 3~10~‐helix. The lactam bridge between the side chains of L‐Glu and L‐Lys in (i) − (i + 3) positions was introduced in order to enhance the structural preference toward the right‐handed 3~10~‐helix. The conformational properties of the three peptides were studied in trifluoroethanol (TFE) solution by CD, NMR, and computer simulations. The structural information was derived mainly from the analysis of nuclear Overhauser effect spectroscopy spectra. The presence of αH(i)—HN(i + 2) and of αH(i)—HN(i + 3) connectivities and the absence of αH(i)—HN(i + 4) connectivities indicate that these peptides fold into a 3~10~‐helix rather than into an α‐helix. Based on these conformational features, stereospecific assignment of the Aib methyl groups was possible. The results of such experiments and of the subsequent distance geometry and restrained molecular dynamics simulations reveal a marked preference of these peptides for 3~10~‐helix. The CD spectra of these peptides indicate that the helix content increases upon chain elongation. The CD spectrum of the trimer is characterized by a negative band at 200 nm and by a weak positive band around 220 nm. The CD spectrum in TFE is different from that observed in aqueous solution in the presence of SDS micelles, reported in our previous work, and from those reported by a different research group for 3~10~‐helical peptides. A possible reason for these differences could rest in the presence of different equilibria of the conformer populations of the various peptides in different solvent systems. © 2005 Wiley Periodicals, Inc. Biopolymers (Pept Sci), 2005