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Conformational Preferences of Peptides Containing Reverse-Turn Mimetic Bicyclic Lactams: Inverse γ-Turns versus Type-II′ β-Turns – Insights into β-Hairpin Stability

✍ Scribed by Laura Belvisi; Cesare Gennari; Antonia Mielgo; Donatella Potenza; Carlo Scolastico


Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
271 KB
Volume
1999
Category
Article
ISSN
1434-193X

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✦ Synopsis


The solid-phase synthesis and characterization of a series of of the reverse-turn mimic. In N-acetylated tetrapeptide mimics incorporating the two different bicyclic lactams (a peptides (3-9), containing reverse-turn mimetic bicyclic lactams (1a, 1b), was reported in the preceding paper. The series and b series), H 5 is available for either a γ-turn (7membered ring with the carbonyl group of the bicyclic bicyclic lactams (1a, 1b) possess high structural similarity to the two central residues of a β-turn. The conformational lactam) or a β-turn (10-membered ring with the carbonyl group of residue 2), as shown in Figures 7 and9. The a series preferences of the constrained peptides have been investigated by NMR spectroscopy and IR spectroscopy. Our incorporating the (5,7)-bicyclic lactam predominantly induces the γ-turn conformation, while the b series experimental results have been complemented by computer modelling studies and show that the constrained peptides (3-incorporating the (5,6)-bicyclic lactam can promote either a γ-turn or a β-turn conformation, with the β-turn usually being 9) form an inverse γ-turn or a type-IIЈ β-turn through intramolecular hydrogen bonding, depending on the nature preferred and with varying degrees of β-hairpin formation.