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Conformational effects in the high-performance liquid chromatography of proteins Further studies of the reversed-phase chromatographic behavior of ribonuclease A

โœ Scribed by X.M. Lu; K. Benedek; B.L. Karger


Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
708 KB
Volume
359
Category
Article
ISSN
1873-3778

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Conformational effects in the reversed-p
โœ S.A. Cohen; K. Benedek; Y. Tapuhi; J.C. Ford; B.L. Karger ๐Ÿ“‚ Article ๐Ÿ“… 1985 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 888 KB

This paper examines the reversed-phase liquid chromatographic behavior of ribonuclease A (RNase) using an n-butyl chemically bonded phase and a gradient of 10 mM H3PO4 and l-propanol. At a column temperature of 25 degrees C, a broad band followed by an overlapped late-eluting sharp peak is observed.