Conformational effects in the reversed-p
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S.A. Cohen; K. Benedek; Y. Tapuhi; J.C. Ford; B.L. Karger
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Article
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1985
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Elsevier Science
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English
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This paper examines the reversed-phase liquid chromatographic behavior of ribonuclease A (RNase) using an n-butyl chemically bonded phase and a gradient of 10 mM H3PO4 and l-propanol. At a column temperature of 25 degrees C, a broad band followed by an overlapped late-eluting sharp peak is observed.