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Conformational dynamics and solvent viscosity effects in carboxypeptidase-A-catalyzed benzoylglycylphenyllactate hydrolysis

โœ Scribed by Nina G. GOGUADZE; Jan M. HAMMERSTAD-PEDERSEN; Dimitrij E. KHOSHTARIYA; Jens ULSTRUP


Book ID
115128735
Publisher
John Wiley and Sons
Year
1991
Tongue
English
Weight
802 KB
Volume
200
Category
Article
ISSN
1432-1327

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N-benzoyl-L-phenylalanyl-L-phenylalanine is an excellent peptide substrate for carboxypeptidase A; at 30ยฐC and pH 7.5, K m is 2.6 ร— 10 -5 M while k cat is 177 s -1 (k cat /K m = 6.8 ร— 10 6 M -1 s -1 ). Indole-3-acetic acid is a noncompetitive or mixed inhibitor towards the peptide and toward hippury