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Conformational comparison of cyclic peptide and pseudopeptide structures with intramolecular hydrogen bonding

✍ Scribed by Arno F. Spatola; Lila M. Gierasch; Arlene L. Rockwell


Publisher
Wiley (John Wiley & Sons)
Year
1983
Tongue
English
Weight
263 KB
Volume
22
Category
Article
ISSN
0006-3525

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✦ Synopsis


An nmr spectral comparison of a model cyclic pentapeptide cyclo(G1y-Pro-Gly-D-Phe-Pro) with an analogous pseudopeptide has been made. The pseudopeptide contains a $[CH2S] amide bond replacement a t the only amide linkage that, in the model, is not involved in an intramolecular hydrogen bond. Both proton and carbon-13 nmr spectral evidence confirms the retention of pand y-turns in the pseudopeptide in chloroform. Characteristic chemical shifts, temperature dependence, and glycine a-resonances support this interpretation. However, evidence of a more flexible conformation involving cis-trans proline isomerism is seen on addition of dimethylsulfoxide.