Conformational aspects of superoxide-dismutase-active copper chelates and catechols

✍ Scribed by Ulrich Weser; Lutz M. Schubotz

• Publisher: Elsevier Science
• Year: 1978
• Weight: 904 KB
• Volume: 9
• Category: Article
• ISSN: 0006-3061
• DOI: 10.1016/s0006-3061(00)80134-9

✦ Synopsis

The pos$ile structxai basis of the specific i&Story action of cuprein on the autoxidation of catfqchola+ne wasexamined. The chjro&al properties -_ of the native enzyme were compared with both tlk denatukd tid the apo-' protein and-i& stiperoxide-disnutasease-active Cu(T$r)p. A&rt froth the reduc-' tion of cuprein copper to Cu(I);marked and chaiact&stic k-es @ ihe -1 circuIar dichroism (CD) spectrum from 260-400 nm jn the-presence bf tidrena-. .I. line and oxygen were seen, suggesti& the. foq&ion of .a ternary compkx.

_.

This conformatikial change proved dep&ae& od the coke&&o~ oi Oxygen, adrenaline, Bnd -cuprein copper and was: not-keen..when the apo&oteti or &heat-denatured enzyme was used.