Conformational aspect of polypeptide structure. XLIV. Conformational transitions of poly(N-methyl-alanines) induced by trifluoroacetic acid

✍ Scribed by Murray Goodman; Fu Chen; Frank R. Prince

• Publisher: Wiley (John Wiley & Sons)
• Year: 1973
• Tongue: English
• Weight: 698 KB
• Volume: 12
• Category: Article
• ISSN: 0006-3525
• DOI: 10.1002/bip.1973.360121109

✦ Synopsis

Abstract

The synthesis of poly(N‐methyl‐L‐alanine) and poly (N‐methyl‐DL‐alanine) are described. The polymers were examined by 220 MHz high‐resolution nuclear magnetic resonance (nmr) and circular dichroism (CD). The results demonstrate that poly(N‐methyl‐L‐alanine) exists as an ordered helical structure with all the amide bonds in the trans configuration in appropriate solvents. As trifluoroacetic acid (TFA) is added to the solutions of the polymer in helix‐supporting solvents, resonances corresponding to both trans and cis amide conformations of N‐methyl, C‐methyl, and α‐CH are observed. The presence of both the trans and the cis peptide bonds in a polymer chain disrupts the ordered structures. Our conclusions from CD data are in agreement with the nmr results. Ultracentrifugation shows that degradation of the polymer chain does not occur during the TFA treatment.