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Conformation of the cyclic tetrapeptide dihydrochlamydocin. Iabu-L-Phe-D-Pro-LX, and experimental values for 3 → 1 intramolecular hydrogen bonds by X-ray diffraction

✍ Scribed by Judith L. Flippen; Isabella L. Karle


Publisher
Wiley (John Wiley & Sons)
Year
1976
Tongue
English
Weight
574 KB
Volume
15
Category
Article
ISSN
0006-3525

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✦ Synopsis


Pro-L,X, is a naturally occurring cyclic tetrapeptide that exhibits high cytostatic activity. The conformation of the peptide ring, as well as the stereo configuration in the vicinity of the epoxide ring, have been established by a single-crystal X-ray study of dihydrochlamydocin: C ~S H ~~N ~O ~H ~O .

It crystallizes in the monoclinic space group P21 with a = 12.616(6) A, b = 12.355(6) A, c = 9.442(5) A, and 6 = 99.5(1)'. The structure was solved by the symbolic addition procedure for phase determination followed by the tangent formula method of phase refinement. This structure represents the first cyclic tetrapeptide in which all four peptide units have been found in the trans conformation; however, each peptide unit is significantly nonplanar with wangles deviating by 14-24' from the ideal value of 180' . This molecule contains two intramolecular 3 -1 hydrogen bonds and experimentally determined parameters for these seven-membered turns are presented.