Conformation of polyriboadenylic acid: pH and temperature dependence
β Scribed by D. N. Holcomb; I. Tinoco Jr.
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1965
- Tongue
- English
- Weight
- 558 KB
- Volume
- 3
- Category
- Article
- ISSN
- 0006-3525
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π SIMILAR VOLUMES
## Abstract The conformation of singleβstranded polyrA in aqueous solution has been measured at temperatures down to β12Β°C. The radius of gyration of lowβmolecularβweight polyrA varies very little with temperature in this range. By studying the dependence of radius of gyration on temperature for se
Raman spectra of solutions of polyriboadenylic acid have been studied in the pH range of 7.2-5.2. Bands are identified which are sensitive to the characteristics of poly(rA) in the singleand double-stranded helical forms. Thermal melting profiles were obtained as a function of pH to monitor simultan
Circular dichroism (CD) curves are reported for poly dA, (pdA),, (pdA)z, poly A, ApAp, ApA, AMP, dApA, pdApA, A-2'4-rnethyl PA, and A-2'4-methyl PAP. Analysis of these curves indicated the presence of single CD bands at 228-230 mp and a t 278-280 mp in oligomers longer than dmucleotides. I n the cas