✦ LIBER ✦

Conformation of double-stranded polydeoxynucleotides in solution by proton two-dimensional nuclear overhauser enhancement spectroscopy

✍ Scribed by Babul Borah; Jack S. Cohen; Ad Bax

Book ID: 102764868
Publisher: Wiley (John Wiley & Sons)
Year: 1985
Tongue: English
Weight: 790 KB
Volume: 24
Category: Article
ISSN: 0006-3525
DOI: 10.1002/bip.360240503

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✦ Synopsis

Proton 2D-NOE spectroscopy has been used to investigate the three-dimensional conformations of several sonicated polydeoxynucleotides in solution. The observed pattern of cross peaks indicate that poly(dAdT) . poly(dAdT1 in all salt concentrations studied (up to 6.6M CsF), and poly(dG-m5dC) . poly(dG-m5dC) in low salt (0.1M NaCU are righthanded B-structures. Poly(dG-m5dC) . poly(dG-m5dC) in Mg2+ (3 mM) solution exhibits a pattern characteristic of the left-handed Z-form. These results for poly(dA-dT) poly(dA-dT) are in contrast to suggestions that this copolymer exists as a left-handed form, either in low or high salt. We present pure absorption-mode ZD-NOE spectra that enable us to compare several distances and define the conformations of these polydeoxynucleotides in solution.

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