Confocal spectral imaging analysis in studies of the spatial distribution of antitumour drugs within living cancer cells
✍ Scribed by S. Sharonov; I. Chourpa; H. Morjani; I. Nabiev; M. Manfait; A. Feofanov
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 724 KB
- Volume
- 290
- Category
- Article
- ISSN
- 0003-2670
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✦ Synopsis
Advanced instrumentation allowing the recording of confocal microfluorescence and micro-Raman spectra, with spatial resolution on the sub-micrometre scale, has been developed . Computer-controlled automatic scanning stage and a system of scanning the laser beam through the stage both permit the recording of well resolved spectral images of samples . Conventional images of species could be recorded with a CCD camera through the same optics . The developed software includes the possibilities of converting a spectral image into a conventional image and of assigning point-by-point the spectral data to the conventional image . This technique was applied to the analysis of the microfluorescent signals from living cancer cells treated with the antitumour drugs doxorubicin and mitoxantrone . It was possible to construct separately the spectral images corresponding to the distribution of drug-DNA complexes, the free drug and intrinsic signals from untreated cell . Consequently, quantitative comparative intracellular and even intranuclear localization of antitumour drugs was achieved . The differences in the intracellular distribution of the drug-DNA complexes inside the cells are discussed in terms of biological effects .