✦ LIBER ✦

Confocal and Atomic Force Microscopy : A Powerful Combination in Cell Research

✍ Scribed by Maarten Balzar; Kate Poole

Publisher: Wiley (John Wiley & Sons)
Year: 2008
Weight: 280 KB
Volume: 10
Category: Article
ISSN: 1439-4243
DOI: 10.1002/imic.200890049

No coin nor oath required. For personal study only.

✦ Synopsis

A wide range of different forms of microscopy may be applied to the visualisation of biological specimens.

Confocal microscopy can provide 3D information about fluorescently labelled cells with the added advantage that it excludes out of focus light. Atomic force microscopy (AFM) provides direct high resolution images of surface features of a sample. Here we look at the microscope configuration necessary for simultaneous confocal and AFM, and examine how the combination of the two techniques together with the 'Direct Overlay' function can be used to great advantage in cell research for correlating surface and internal features.

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