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Conductometric urea sensor. Use of additional membranes for the improvement of its analytical characteristics

✍ Scribed by A.S. Jdanova; S. Poyard; A.P. Soldatkin; N. Jaffrezic-Renault; C. Martelet

Publisher: Elsevier Science
Year: 1996
Tongue: English
Weight: 362 KB
Volume: 321
Category: Article
ISSN: 0003-2670
DOI: 10.1016/0003-2670(95)00548-x

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✦ Synopsis

Urea-sensitive enzymatic conductometrie biosensors based on interdigitated gold electrodes were prepared by cross-linking urease with bovine serum albumin in a saturated glutaral~hyde valmur on the sensor chips. Atklitional Nation or PVP (poly (4-vinylpyridine-co-styrene)) membranes were deposited on top of the urea mlcrnsensor by a spin-coating process. The main analytical characteristics (dynamic range, bufth" concentration sensitieJty) of these urea-sensitive microcondactometric devices were not significantly modified by the use of Nation additional membranes. BoRer results were obtained for sonsms coated with PVP additional membranes: their operational storage stability and reproducibility are promising for further applications, especially in the biomedical field.

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