Conditions for the measurement of quantitative off-resonance ROESY intensities

✍ Scribed by T. E. Malliavin; H. Desvaux; M.-A. Delsuc

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 255 KB
• Volume: 36
• Category: Article
• ISSN: 0749-1581
• DOI: 10.1002/(sici)1097-458x(1998110)36:11<801::aid-omr367>3.0.co;2-j

✦ Synopsis

Two phenomena hamper the quantiÐcation of ROESY intensities : the o †-resonance e †ect and the coherent magnetization transfer between scalar-coupled spins. The o †-resonance application of the r.f. irradiation has been proposed to reduce these artefacts. Here the reliability of this method for the quantiÐcation of ROESY intensities measured on a sample containing a macromolecule was investigated. The threshold of irradiation o †set for which the coherent magnetization transfer can be neglected was determined. It is shown that for o †sets larger than this threshold, the o †-resonance e †ect does not hinder the quantiÐcation, provided that the irradiation is alternatively applied at high and low Ðeld.