Conditional deletion of the colony stimulating factor-1 receptor (c-fms proto-oncogene) in mice

✍ Scribed by Jia Li; Ken Chen; Liyin Zhu; Jeffrey W. Pollard

• Publisher: John Wiley and Sons
• Year: 2006
• Tongue: English
• Weight: 526 KB
• Volume: 44
• Category: Article
• ISSN: 1526-954X
• DOI: 10.1002/dvg.20219

✦ Synopsis

Colony stimulating factor-1 (CSF-1) is the primary regulator of the mononuclear phagocytic lineage acting through its transmembrane tyrosine kinase receptor, CSF-1R, that is the product of the c-fms proto-oncogene. Null mutations in either the ligand or the receptor genes result in a severe osteopetrosis as well as a number of other phenotypes, including reproductive defects and perturbations in organ development. The CSF-1R is also expressed in oocytes, myoblast progenitors, decidual, and trophoblastic cells. To distinguish cell type specific phenotypes, we have created a conditional allele of the Csf1r by placing LoxP sites around Exon 5 of the Csf1r gene in mice. Excision of this floxed sequence results in a null allele that in the homozygous state gives a phenotype indistinguishable of the complete Csf1r null mutant mouse. This conditional allele will prove extremely valuable to study the spatial and temporal roles of CSF-1R.