Conditional and replicative senescence in Escherichia coli
✍ Scribed by Åsa Fredriksson; Thomas Nyström
- Book ID
- 116747397
- Publisher
- Elsevier Science
- Year
- 2006
- Tongue
- English
- Weight
- 315 KB
- Volume
- 9
- Category
- Article
- ISSN
- 1369-5274
No coin nor oath required. For personal study only.
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When E. coli F+ cells carrying the dna-167 or dnaC2 mutation, which causes the temperature-sensitive initiation of DNA replication, are exposed to a non-permissive temperature to stop the replication of chromosome and F factor, and then transferred back to a permissive temperature with the addition
Temperature-sensitive mutants defective in the initiation of DNA replication are exposed to a non-permissive temperature to complete already initiated replication, and are transferred back to a permissive temperature. DNA synthesis can resume in the presence of rifampicin or rifampicin plus chloramp