Condensed E. coli cultures for highly efficient production of proteins containing unnatural amino acids

✍ Scribed by Jia Liu; Carlos A. Castañeda; Bryan J. Wilkins; David Fushman; T. Ashton Cropp

• Publisher: Elsevier Science
• Year: 2010
• Tongue: English
• Weight: 482 KB
• Volume: 20
• Category: Article
• ISSN: 0960-894X
• DOI: 10.1016/j.bmcl.2010.08.049

✦ Synopsis

Current biosynthetic methods for producing proteins containing site-specifically incorporated unnatural amino acids are inefficient because the majority of the amino acid goes unused. Here we present a universal approach to improve the efficiency of such processes using condensed Escherichia coli cultures.