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Concurrent quantification of tissue metabolism and blood flow via 2h/31P NMR in Vivo. 11. validation of the deuterium nmr washout method for measuring organ perfusion

✍ Scribed by Jeffrey J. Neil; Sheng-kwei Song; Joseph J. H. Ackerman

Publisher
John Wiley and Sons
Year
1992
Tongue
English
Weight
710 KB
Volume
25
Category
Article
ISSN
0740-3194

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✦ Synopsis

Abstract

The deuterium washout technique of measuring tissue blood flow is based upon NMR detection of HOD (administered as D~2~O saline, but typically detected as HOD because of rapid proton‐deuteron exchange) as a freely diffusible tracer. Though this method is coming into more general use, it has not yet been rigorously validated in vivo against an accepted, independent measure of tissue blood flow. To this end, simultaneous radiolabeled micro‐sphere and HOD washout blood flow measurements were made in rat gastrocnemius muscle. D~2~O saline was administered either intramuscularly or intraarferially (near the aortic bifurcation), and the sciatic nerve was electrically stimulated to increase the muscle blood flow rate. Over a range of flows of 2 to 80 ml/(100 g. min), comparison of micro‐sphere and HOD washout measurements showed good agreement, with r = 0.92 (n = 16) for intramuscular administration and r = 0.91 (n = 12) for intraarterial administration. These data strongly suggest thatthe HOD washout technique provides accurate blood flow measurements in skeletal muscle.

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