✦ LIBER ✦
Concomitant purification of three porcine heart mitochondrial enzymes: Citrate synthase, aspartate aminotransferase, and malate dehydrogenase
✍ Scribed by Arthur J. McEvily; Andrew J. Flint; John H. Harrison
- Publisher
- Elsevier Science
- Year
- 1985
- Tongue
- English
- Weight
- 421 KB
- Volume
- 144
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
The mitochondrial enzymes citrate synthase, malate dehydrogenase, and aspartate aminotransferase were purified to homogeneity from porcine hearts by use of Bio-Rex 70, carboxymethylcellulose CM32, and Affi-Gel blue chromatography. This procedure provides relatively rapid, large-scale preparation of the three enzymes based on their differential binding to commercially available cation-exchange resins followed by a final affinity chromatography step.