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Concentration profiles in free-flow electrophoresis

✍ Scribed by Joaquim F. G. Reis; E. N. Lightfoot; Ho-Lun Lee

Publisher
American Institute of Chemical Engineers
Year
1974
Tongue
English
Weight
663 KB
Volume
20
Category
Article
ISSN
0001-1541

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A flow cytometric assay has been developed for determination of intracellular free potassium concentration ([K 1 ] i ). Investigated cells, loaded with the fluorescent pH indicator 28,7bis-(2-carboxyethyl)-5(and-6)-carboxyfluorescein (BCECF), are incubated in the presence of nigericin, and the intra