Concentration of mast-cell progenitors in bone marrow, spleen, and blood of mice determined by limiting dilution analysis

✍ Scribed by Takashi Sonoda; Takako Ohno; Yukihiko Kitamura

• Publisher: John Wiley and Sons
• Year: 1982
• Tongue: English
• Weight: 512 KB
• Volume: 112
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1041120120

✦ Synopsis

Abstract

When hematopoietic cells of congenic +/+ mice were injected into the skin of genetically mast‐cell‐depleted (WB × C57BL/6)F~1~‐W/W^v^ mice, mast cells appeared at the injection site. The donor origin of developing mast cells was confirmed by using granules of C57BL/6‐bgl/bgl mice as a marker. When the number of injected cells was decreased, the proportion of injection sites at which mast cells did not appear increased according to the expected frequency of null response in a Poisson distribution. Therefore, such proportions were used to calculate the concentration of mast‐cell precursors in the bone marrow, spleen, and peripheral blood. The relative concentration of mast‐cell precursors in these tissues was similar to that of spleen‐colony‐forming cells. The present method seems useful as a semiquantitative in vivo assay for a population of progenitor cells which are committed to differentiate into mast cells.