Concentration of autoantibodies to native 60-kd ro/ss-a and denatured 52-kd ro/ss-a in eluates from the heart of a child who died with congenital complete heart block
✍ Scribed by Morris Reichlin; Antonio Brucato; Mark B. Frank; Peter J. Maddison; Victoria R. Mccubbin; Marianne Wolfson-Reichlin; Lela A. Lee
- Publisher
- John Wiley and Sons
- Year
- 1994
- Tongue
- English
- Weight
- 558 KB
- Volume
- 37
- Category
- Article
- ISSN
- 0004-3591
No coin nor oath required. For personal study only.
✦ Synopsis
Objective:
To determine the serologic specificity of acid eluates from tissues of a child who died with congenital complete heart block (cchb).
Methods:
Tissues were extracted, acid eluted, and the igg and antibody titers determined on the eluates by enzyme-linked immunosorbent assay.
Results:
Antibodies to native 60-kd and denatured 52-kd ro/ss-a were found to be enriched only in the heart eluate, and not in the eluates from brain, kidney, and skin.
Conclusion:
These findings indicate a major role for anti-native 60-kd ro/ss-a in the immunopathogenesis of cchb.