✦ LIBER ✦

Concentration of activated t lymphocytes in extracorporeal blood circulation for plasma separation

✍ Scribed by Shinji Ijichi; Masaki Mishima; Takemasa Matsuda; Tomomaro Izumihara; Kazuhisa Kojima; Tatsuru Niimura; Ikuro Maruyama; Mitsuhiro Osame

Publisher: John Wiley and Sons
Year: 1991
Tongue: English
Weight: 202 KB
Volume: 6
Category: Article
ISSN: 0733-2459
DOI: 10.1002/jca.2920060206

No coin nor oath required. For personal study only.

✦ Synopsis

To investigate whether the proportion of activated T lymphocytes changes in an extracorporeal blood flow, we compared paired samples collected at the inlet and outlet lines of an artificial circuit for plasma separation, using a dual-immunofluorescence flow cytometric technique. In our series of materials from patients with rheumatoid arthritis or systemic lupus erythematosus, the percentages of HLA-DR-positive cells in both CD4+ ( P < 0.01, n = 6 ) and CD8' ( P < 0.05, n = 10) subsets were significantly increased in the outlet lines compared with the inlet lines, suggesting that activated T lymphocytes pass through an extracorporeal blood circuit more easily than resting T lymphocytes.

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