Concentration Measurement of Unpurified Proteins Using Biosensor Technology under Conditions of Partial Mass Transport Limitation

✍ Scribed by Pascale M. Richalet-Sécordel; Nathalie Rauffer-Bruyère; Lars L.H. Christensen; Beatus Ofenloch-Haehnle; Christoph Seidel; Marc H.V. Van Regenmortel

• Publisher: Elsevier Science
• Year: 1997
• Tongue: English
• Weight: 150 KB
• Volume: 249
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1006/abio.1997.2183

✦ Synopsis

tion that they do not distinguish between biologically Using biosensor technology, it is possible to measure active and inactive molecules. Both of these limitations protein concentration when the binding of the protein can be overcome by determining protein concentration to an appropriate ligand immobilized on the sensor with biosensor instruments based on surface plasmon surface is totally limited by diffusion and mass transresonance (SPR). 2 cable to unpurified samples and suffer from the limita-TMVP, tobacco mosaic virus coat protein; NHS, N-hydroxysuccinimide; EDC, N-ethyl-N-dimethylaminopropyl carbodiimide; HBS, Hepes-buffered saline; PDEA, 2-(2-pyridinyldithioethaneamine);