Concentration effects on partitioning of dextrans and serum albumin in porous glass

✍ Scribed by Brannon, J. H. ;Anderson, J. L.

• Publisher: Wiley (John Wiley & Sons)
• Year: 1982
• Tongue: English
• Weight: 460 KB
• Volume: 20
• Category: Article
• ISSN: 0098-1273
• DOI: 10.1002/pol.1982.180200508

✦ Synopsis

Abstract

Partitioning of three dextrans and bovine serum albumin (BSA) in controlled‐pore glass beads was measured by a batch mass balance technique. For all solutes the measured partition coefficient increased with bulk phase concentration, and the relationship appeared to be linear up to 0.05 g/mL for the dextrans. The measured slopes of partition coefficient versus concentration were compared with predictions using a hard‐sphere theory, and while the agreement was tolerable for BSA, the dextran results were much lower than the theory dictated except for very small pores, in which case it was speculated that molecular weight polydispersity resulted in anomalous partitioning of the smaller components.