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Concentration and partial purification of lipase fromPseudomonas fluorescens

✍ Scribed by R. E. Andersson

Publisher
Springer Netherlands
Year
1980
Tongue
English
Weight
285 KB
Volume
2
Category
Article
ISSN
0141-5492

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The e-glucosidase (C~-D-glucoside glucohydrolase, EC 3.2.1.20) of Pseudomonas fluorescens W was partially purified by (NH4)2SO4 fractionation, Sepha-dexG-200 and DEAE-cellulose column chromatography. The enzyme showed great specificity for maltose hydrolysis, with very little action against polymeri